Ammonia Analysis in Seawater with Fluorimetric Detection

biological activities in marine environments

Ammonia plays a significant role in biological activities in marine environments and is frequently analyzed by many laboratories and marine institutes worldwide. For many years Skalar successfully automates the ammonia method according the ISO and EPA directives, allowing the measurement of ppb levels of ammonia.

Specifically for the analysis of ammonia in seawater, Skalar is pleased to introduce a newly developed method based on fluorimetric detection. This method is becoming more and more accepted and is particularly interesting because of its simplicity. Also fewer reagents are required and furthermore there is no compromise on detection limits. The newly developed method is based upon the reaction of ammonia with ortho-phtaldialdehyde (OPA) and sulfite, which produces an intense fluorescent product. This is measured by a fluorimeter with an excitation wavelength of 370 nm and an emission wavelength range of 418-700 nm.

San++ Automated Wet Chemistry Analyzer

Skalar has successfully integrated this new ammonia method in the SAN++ analyzer in combination with the sensitive Skalar SA6310 Fluorimeter. The typical analysis range is 1- 100 ppb Ammonia with detection limits in the sub ppb levels.

Below you can find the real-time view and calibration curve of the fluorimetric ammonia method in the range up to 100 ppb.

Real-time view of the fluorimetric ammonia method Calibration curve of the fluorimetric ammonia method

The flexibility of the SAN++ analyzer and Skalar's vast knowledge and experience in automatic wet-chemistry analysis, provide all our customers worldwide reliable and accurate automation solutions for a variety of applications.

If you require more information on the automation of Ammonia method or other Skalar methods, do not hesitate to contact us.