Protein determination in collagen samples

Michele Lanoue, Reagents Business Team Lead with
the Skalar Formacs Total Nitrogen Analyzer

Currently Becton Dickinson BioSciences Discovery Labware in Bedford, Massachusetts is using the Formacs as a dedicated Total Nitrogen (TN) analyzer, utilizing the chemiluminescent detector, for determining the percentage of protein found in their collagen samples. They required that the Skalar Formacs TN analyzer was able to analyze from 0.625 mg/L of N to 20 mg/L of N with a very high accuracy and precision.

Using the Formacs TN analyzer, analysis in the above mentioned range is achieved, with coefficient of variance (C.V.) values of less or equal to 1% within two injections and never more than three injections. The Formacs TN results correlated very well with the analyzer they had been using before, making their changeover to the Skalar Formacs TN analyzer easy.

The protein value gives Becton Dickinson a quality control value, which they use as an indicator if their collagen is acceptable. Below they have provided a summation explaining the importance of their work in the scientific community. They provide collagen for there clients to carry out much needed research throughout the medical research community.

In order to create physiologically relevant in vitro models that support normal cell growth and function, the components of the in vivo environment must be incorporated. Use of extra cellular matrix (ECM) as a coating for tissue culture surfaces permits the development of model systems, which closely mimic in vivo conditions. The choice of ECM is an important component to consider when optimizing in vitro culture systems.

Michele Lanoue and Skalar’s application chemist Al Yates

Collagen I can be used as a thin layer on tissue-culture surfaces to enhance cell attachment and proliferation, or as a gel to promote expression of cell-specific morphology and function. Type 1 collagen is commonly used to culture endothelial cells, hepatocytes, muscle cells, and a variety of other cell types.

Collagen IV is a component of basement membranes, the sheet-like matrix, which underlies epithelial and endothelial cells and surrounds muscle fat and nerve cells. It can be used as a thin coating on tissue culture surfaces to promote cell attachment and proliferation and to study its effects on cell behavior. Type IV Collagen is commonly used to culture PC12 cells and neuronal cells.

Below is the link to BD’s ECM home page for more information on their products:

For more information on the Skalar Formacs TN analyzer and other Skalar products, please contact Skalar Analytical BV